polyclonal ab against intermediate peptide (Santa Cruz Biotechnology)
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polyclonal ab against intermediate peptide
Polyclonal Ab Against Intermediate Peptide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+ab+against+intermediate+peptide/Intermediate/pm10409614-59-1-25
Average 93 stars, based on 94 article reviews
Polyclonal Ab Against Intermediate Peptide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+ab+against+intermediate+peptide/Intermediate/pm10409614-59-1-25
Average 93 stars, based on 94 article reviews
polyclonal ab against intermediate peptide - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40."
Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40.
Journal: The Journal of biological chemistry
doi: 10.1074/jbc.274.30.20759
Figure Legend Snippet: FIG. 2. Both RNA transcripts and proteins of DFF35/45 exist in vivo. A, DFF35 and DFF45 were amplified by human DFF35 (lanes 1 and 3) and DFF45 (lanes 2 and 4) out-encoding region primer sets in human fetal liver cDNA (lanes 1 and 2) or Jurkat cell line (lanes 3 and 4) using PCR. B, expression of DFF45 and DFF35 in Jurkat cells was immunoblotted by polyclonal Ab specific for N-terminal (lane 1), inter- mediate (lane 2), or C-terminal (lane 3), respectively. C, lysate of 293T cells transfected with DFF45 (lane1), DFF35 (lane 2), or an irrelevant protein p53 (lane 3) was immunoblotted by anti-Flag (M5) Ab (top panel), or anti DFF45/35 (bottom panel). Arrows indicate the trans- fected and endogenous DFF45 and DFF35.
Techniques Used: In Vivo, Amplification, Expressing, Transfection
Figure Legend Snippet: FIG. 1. Comparison of amino acid sequences between DFF35 and DFF45 (A) or mouse ICAD-S (B). Two putative cleavage sites for caspase-3 are indicated by double underlines. Three peptides (N, I, and C) used to generate polyclonal antibodies are indicated by single under- lines. Identical amino acids between DFF35 and mouse ICAD-S are in bold face.
Techniques Used: Comparison
Figure Legend Snippet: FIG. 3. Inhibitory effect of DFF35 in vitro and in vivo. A, Jurkat cells were treated with 1 mg/ml Fas monoclonal Ab 7C11 for 2 h, and 100 ng of cytosolic fraction without (lane 1) or with GST (lanes 2 and 3), GST-DFF35 (lanes 4 and 5), or double mutated GST-DFF35 (lanes 6 and 7) was added to Jurkat nuclei and incubated at 37 °C for 2 h in the presence (lanes 3, 5, and 7) or absence (lanes 1, 2, 4, and 6) of caspase-3, respectively. Jurkat cells were treated with 1 mg/ml of staurosporine for the indicated time, cell lysates were blotted with polyclonal Ab reactive to N-terminal peptide of DFF45 and DFF35 (B), and genomic DNA were extracted and subjected to analysis by 2% agarose gel electrophoresis (C).
Techniques Used: In Vitro, In Vivo, Incubation, Agarose Gel Electrophoresis
Related Articles
In Vivo:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A Amplification:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A Expressing:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A Transfection:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A Comparison:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A In Vitro:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A Incubation:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A Agarose Gel Electrophoresis:Article Title: Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40. Article Snippet: Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.Western Blot Analysis—A polyclonal Ab against the N-terminal peptide of both DFF35 and DFF45 (EVTGDAGVPESGEIRTLPKC) was from Upstate Biotechnology.. A |